FIXATION IN CYTOCHEMISTRY AND ELECTRON-MICROSCOPY

Fixation in cytochemistry and electron-microscopy.

Whenever it is our intention to study objects that are visible with the light-microscope, it is reasonable to start with the living cell, to study the effects of fixation on its visible constituents, to analyse their composition by cytochemical methods, and then to explore their fine structure by the use of the electron microscope. A manysided attack of this sort on a single kind of cell is muc...

Cytochemistry and Electron Microscopy

THE ALDEHYDES INTRODUCED IN THIS PAPER AND THE MORE APPROPRIATE CONCENTRATIONS FOR THEIR GENERAL USE AS FIXATIVES ARE: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylate- or phosphate-buffered solutions (0.1 to 0....

CYTOCHEMISTRY AND ELECTRON MICROSCOPY The Preservation of Cellular Ultrastructure and Enzymatic Activity by Aldehyde Fixation

The aldehydes introduced in this paper and the more appropriate concentrations for their general use as fixatives are: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylateor phosphate-buffered solutions (0.1 to 0.2 ...

CYTOCHEMISTRY AND ELECTRON MICROSCOPY The Preservation of Cellular Ultrastructure and Enzymatic Activity by Aldehyde Fixation

The aldehydes introduced in this paper and the more appropriate concentrations for their general use as fixatives are: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylateor phosphate-buffered solutions (0.1 to 0.2 ...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test